Kedu usoro kacha mma?
-Nnwale maka nchọpụta ọrịa SARS-CoV-2
Maka ikpe COVID-19 enwetara ekwenyesiri ike, a kọrọ na mgbaàmà ụlọ ọgwụ a na-ahụkarị gụnyere ahụ ọkụ, ụkwara, myalgia ma ọ bụ ike ọgwụgwụ.Agbanyeghị akara ngosi ndị a abụghị ihe pụrụ iche nke COVID-19 n'ihi na akara ngosi ndị a yiri nke ọrịa ndị ọzọ bu nje dị ka influenza.Ugbu a, nje nucleic acid Real-Time PCR (rt-PCR), CT imaging na ụfọdụ hematology paramita bụ isi ngwaọrụ maka nyocha ụlọ ọgwụ nke ọrịa ahụ.Emepụtala ọtụtụ ngwa nyocha ụlọ nyocha wee jiri ya na-anwale ụdị ndị ọrịa maka COVID-19 nke China CDC.1, US CDC2na ụlọ ọrụ nzuzo ndị ọzọ.Nnwale mgbochi IgG/IgM, usoro nnwale serological, ka agbakwunyere dị ka usoro nyocha na ụdị China emelitere nke nyocha na ntuziaka ọgwụgwọ maka ọrịa coronavirus ọhụrụ (COVID-19), nke ewepụtara na 3rd, Maachị.1.Nnwale nje nucleic acid rt-PCR ka bụ usoro nchọpụta ọkọlọtọ ugbu a maka nyocha nke COVID-19.
Nzọụkwụ siri ike®Novel Coronavlrus (SARS-COV-2) Ngwa PCR Real-Time Multiplex (nchọpụta maka mkpụrụ ndụ ihe nketa atọ)
Ma ngwa ule PCR ndị a dị adị, na-achọ ihe mkpụrụ ndụ ihe nketa nke nje, dịka ọmụmaatụ na imi, ọnụ, ma ọ bụ gbasara swabs, na-enwe ọtụtụ oke:
1) Nnwale ndị a nwere ogologo oge ntụgharị na mgbagwoju anya na arụ ọrụ;ha na-ewekarị ihe karịrị awa 2 ruo 3 iji mepụta nsonaazụ.
2) Nnwale PCR chọrọ ụlọ nyocha akwụkwọ ikike, akụrụngwa dị oke ọnụ na ndị ọrụ nka zụrụ azụ ka ha rụọ ọrụ.
3) Enwere ụfọdụ ọnụọgụ ụgha ụgha maka rt-PCR nke COVID-19.Ọ nwere ike n'ihi obere nje virus SARS-CoV-2 dị n'ụdị swab iku ume elu (Novel coronavirus na-ebutekarị traktị akụkụ okuku ume, dị ka pulmonary alveoli) na nnwale ahụ enweghị ike ịchọpụta ndị butere ọrịa, gbakere, na kpochapụrụ nje ahụ n'ahụ ha.
Nnyocha nke Lirong Zou et al4achọpụtara na achọpụtara ibu nje virus dị elu n'oge na-adịghị anya ka akara ngosi ahụ malitere, ebe a na-achọpụta ibu nje dị elu na imi karịa na akpịrị yana usoro nje nucleic acid na-agbapụta nke ndị ọrịa butere SARS-CoV-2 yiri nke ndị ọrịa nwere influenza.4ma na-apụta dị iche na nke a na-ahụ na ndị ọrịa butere SARS-CoV-2.
Yang Pan et al5Nyochaa usoro ihe atụ (swabs akpịrị, sputum, mmamịrị na stool) sitere na ndị ọrịa abụọ nọ na Beijing wee chọpụta na ibu nje na akpịrị akpịrị na sputum sample ruru n'ihe dị ka ụbọchị 5-6 mgbe akara ngosi malitere, ihe nlele sputum n'ozuzu gosipụtara ibu nje dị elu karịa. akpịrị swab sample.Ọ nweghị nje RNA ahụrụ na mmamịrị ma ọ bụ stool sample sitere n'aka ndị ọrịa abụọ a.
Nnwale PCR na-enye nsonaazụ dị mma naanị mgbe nje ka dị.Nnwale ndị ahụ enweghị ike ịmata ndị butere ọrịa, gbakere ma kpochapụ nje ahụ n'ahụ ha.N'ezie, naanị ihe dị ka 30% -50% dị mma maka PCR na ndị ọrịa nwere ọrịa oyi baa coronavirus achọpụtara n'ụlọ ọgwụ.Ọtụtụ ndị ọrịa oyi baa coronavirus enweghị ike ịchọpụta n'ihi ule nucleic acid na-adịghị mma, yabụ na ha enweghị ike nweta ọgwụgwọ kwekọrọ na oge.Site na nke mbụ ruo mbipụta nke isii nke ụkpụrụ nduzi, ịdabere naanị na nyocha nke nsonaazụ nyocha nke nucleic acid, bụ nke kpatara nnukwu nsogbu nye ndị dọkịta. "Onye mbụ na-agba ọkụ", Dr. Li Wenliang, onye na-ahụ maka anya na Wuhan Central. Ụlọ ọgwụ, anwụọla.N'oge ndụ ya, o nwere ule nucleic acid atọ n'ihe banyere ahụ ọkụ na ụkwara, na oge ikpeazụ ọ nwetara PCR nti utịp.
Mgbe ndị ọkachamara kparịtachara ụka, e kpebiri ịbawanye ụzọ nnwale ọbara dị ka akara nyocha ọhụrụ.Ọ bụ ezie na a na-anwale ọgwụ mgbochi mmadụ, nke a na-akpọkwa ule serological, nke nwere ike igosi ma mmadụ butere ọrịa ọbụlagodi mgbe usoro ahụ ji alụso ọrịa ọgụ kpochapụrụ nje na-akpata COVID-19.
StrongStep® SARS-COV-2 IgG/IgM Antibody Rapid Test
Nnwale mgbochi IgG/IgM ga-enyere aka ịchọpụta n'ụzọ ndị ọzọ dabere na ndị butere ọrịa ahụ, n'ihi na ọtụtụ ikpe yiri ka a na-agbasa site na ndị ọrịa asymptomatic na-enweghị ike ịmata ngwa ngwa.Otu di na nwunye na Singapore, di ahụ nwalere ihe ziri ezi site na PCR, nsonaazụ PCR nwunye ya adịghị mma, mana nsonaazụ nyocha nke mgbochi mmadụ gosiri na o nwere ọgwụ mgbochi ọrịa, dị ka di ya nwere.
Ekwesịrị iji nlezianya kwado nyocha nke serological iji jide n'aka na ha na-emeghachi omume na ntụkwasị obi, mana ọ bụ naanị ọgwụ mgbochi megide nje ọhụrụ.Otu ihe na-echegbu bụ na myirịta dị n'etiti nje na-akpata nnukwu ọrịa iku ume na COVID-19 nwere ike ibute mmeghachi omume.IgG-IgM mebere Xue Feng wang6a na-ewere na enwere ike iji ya mee ihe dị ka ule nyocha (POCT), n'ihi na enwere ike ime ya n'akụkụ ihe ndina na ọbara mkpịsị aka.Ngwa ahụ nwere uche nke 88.66% yana nkọwapụta nke 90.63%.Agbanyeghị, a ka nwere nsonaazụ dị mma na ụgha ụgha.
Na ụdị nyocha nyocha na ntuziaka ọgwụgwọ nke China emelitere maka ọrịa coronavirus ọhụrụ (COVID-19)1, akọwara ikpe enwetara dị ka ndị a na-enyo enyo na ha mezuru otu n'ime usoro ndị a:
(1) Ihe nlele traktị iku ume, ọbara ma ọ bụ stool a nwalere ihe dị mma maka SARS-CoV-2 nucleic acid site na iji rt-PCR;
(2) Usoro mkpụrụ ndụ ihe nketa nke nje sitere na traktị iku ume, ọbara ma ọ bụ ihe nlele stool bụ ihe jikọrọ ya na SARS-CoV-2 a ma ama;
(3) Serum novel coronavirus kpọmkwem mgbochi IgM na mgbochi IgG dị mma;
(4) Serum novel coronavirus-kpọmkwem IgG mgbochi gbanwere site na-adịghị mma gaa na nke ọma ma ọ bụ coronavirus kpọmkwem IgG mgbochi n'oge mgbake dị okpukpu anọ karịa nke ahụ n'oge nnukwu oge.
Nchọpụta na ọgwụgwọ nke COVID-19
| Ntuziaka | Ebipụtara ya | Ekwenyere njirisi nyocha |
| Ụdị nke asaa | 3 Maachị 2020 | PCR ❷ NGS ❸ IgM+IgG |
| Ụdị nke 6 | Febụwarị 18.2020 | PCR ❷ NGS |
Ntụaka
1. Ntuziaka maka nchoputa na ọgwụgwọ nke novel coronavirus oyi baa (ụdị nnwale 7, National Health Commission of the People's Republic of China, nyere na 3.Mar.2020)
http://www.nhc.gov.cn/yzygj/s7652m/202003/a31191442e29474b98bfed5579d5af95.shtml
2. Jiri nyocha naanị ezigbo oge RT-PCR Protocol maka njirimara 2019-nCoV
https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html
3. Singapore na-ekwu na ojiji mbụ e mere ọgwụ mgbochi iji chọpụta ọrịa coronavirus
https://www.sciencemag.org/news/2020/02/singapore-claims-first-use-antibody-test-track-coronavirus-infections
4.SARS-CoV-2 Viral Ibu Ibu n'ụdị iku ume elu nke ndị ọrịa butere February 19,2020 DOI: 10.1056/NEJMc2001737
5.Viralloads nke SARS-CoV-2 na nlele ụlọ ọgwụ Lancet Infect Dis 2020 Bipụtara na ntanetị February 24, 2020 (https://doi.org/10.1016/S1473-3099(20)30113-4)
6. Ngwa mmepe na ụlọ ọgwụ nke ngwa ngwa IgM-IgG Ngwakọta mgbochi mgbochi maka SARS-CoV-2
Nchọpụta ọrịa XueFeng Wang ORCID iD: 0000-0001-8854-275X
Oge nzipu: Mar-17-2020
