Nnwale ngwa ngwa PROM
Ezubere iji
Nzọụkwụ siri ike®Nnwale PROM bụ nyocha anya, qualitative immunochromatographic ule maka nchọpụta IGFBP-1 sitere na mmiri amniotic na nzuzo nzuzo n'oge ime.Ezubere ule a maka iji ndị ọkachamara nyere aka chọpụta mgbawa nke membranes nwa ebu n'afọ (ROM) n'ime ụmụ nwanyị dị ime.
Okwu mmalite
Nleta nke IGFBP-1 (insulin-dị ka ihe na-eto eto na-ejikọta protein-1) n'ime mmiri ọmụmụ bụ 100 ruo 1000 ugboro dị elu karịa na ọbara nne.IGFBP-1 anaghị adịkarị na ikpu, ma mgbe mgbawa nke akpụkpọ anụ nwa ebu n'afọ, mmiri ọmụmụ nke nwere oke IGFBP-1 na-ejikọta ya na nzuzo nzuzo.N'ule StrongStep® PROM, a na-ewere swab polyester na-adịghị ọcha were were were were swab swab na-ewere ihe nlere nke nzuzo nke ikpu wee wepụta ihe nlere ahụ n'ime Ngwọta Ngwọta Mwepụta.A na-achọpụta ọnụnọ nke IGFBP-1 na ngwọta site na iji ngwaọrụ nyocha ngwa ngwa.
Ụkpụrụ
Nzọụkwụ siri ike®Nnwale PROM na-eji ụcha immunochromatographic, teknụzụ na-erugharị capillary.Usoro ule na-achọ solubilization nke IGFBP-1 site na swab ikpu site na ịgwakọta swab na Sample Buffer.Mgbe ahụ, a na-agbakwunye ihe nchekwa ihe agwakọtara na kaseti nlele nke ọma na ngwakọta na-akwaga n'akụkụ elu akpụkpọ ahụ.Ọ bụrụ na IGFBP-1 dị na sample, ọ ga-etolite a mgbagwoju na isi mgbochi IGFBP-1 mgbochi conjugated ka acha ahụ.A ga-ejikọta mgbagwoju ahụ site na mgbochi IGFBP-1 nke abụọ nke ekpuchiri na akpụkpọ ahụ nitrocellulose.Ọdịdị nke akara ule a na-ahụ anya yana akara akara ga-egosi nsonaazụ dị mma.
Ngwakọta ngwa
| 20 Onye ọ bụla pngwaed ule ngwaọrụ | Ngwaọrụ ọ bụla nwere eriri nwere conjugates nwere agba yana reactive reactive ndị ekpuchiri mbụ na mpaghara ndị kwekọrọ. |
| 2mmịpụtaIhe nchekwa vial | 0.1M Phosphate buffered saline (PBS) na 0.02% sodium azide. |
| 1 swab njikwa dị mma (na arịrịọ naanị) | Nwere IGFBP-1 na sodium azide.Maka njikwa mpụga. |
| 1 swab njikwa na-adịghị mma (na arịrịọ naanị) | Enweghị IGFBP-1.Maka njikwa mpụga. |
| 20 Ọkpụkpụ amịpụta | Maka ụdị nkwadebe eji. |
| 1 Ụlọ ọrụ | Ebe maka ijide vial na tubes. |
| 1 Ntinye ngwugwu | Maka ntuziaka ọrụ. |
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
| Ngụ oge | Maka iji oge. |
MKPACHAPỤ ANYA
∎ Maka ọkachamara na-achọpụta ọrịa in vitro naanị.
∎ Ejila ụbọchị njedebe nke egosiri na ngwugwu ahụ.Ejila ule ma ọ bụrụ na akpa foil ya mebiri emebi.Ejighachila ule ọzọ.
■ Ngwa a nwere ngwaahịa sitere na anụmanụ.Ọmụma akwadoro maka mmalite na/ma ọ bụ ọnọdụ ịdị ọcha nke anụmanụ anaghị ekwe nkwa kpamkpam enweghị ihe nje na-efe efe.Ya mere, a na-atụ aro ka a na-emeso ngwaahịa ndị a dị ka ndị nwere ike ibute ọrịa, ma jikwaa ya na-edebe nchekwa nchekwa ndị a na-emebu (a etinyela ma ọ bụ kuku ume).
∎ Zenarị mmetọ nke ihe nlere anya site n'iji igbe nnakọta ihe ọhụrụ maka ụdịdị ọ bụla enwetara.
■ Jiri nlezianya gụọ usoro niile tupu ịme ule ọ bụla.
∎ erila, ṅụọ ihe ọṅụṅụ ma ọ bụ sịga n'ebe a na-edozi ihe nlele na ngwa.Jikwaa ụdịdị niile dị ka a ga-asị na ha nwere ihe na-efe efe.Leba anya n'akpachapụ anya siri ike megide ihe egwu microbiological n'ime usoro a niile wee soro usoro ọkọlọtọ maka mkpofu nke ọma.Yiri uwe nchebe dị ka uwe nyocha ụlọ nyocha, gloves enwere ike iwepu na nchedo anya mgbe a na-enyocha ihe nlele.
∎ Agbanwela ma ọ bụ gwakọta reagents site na nza dị iche iche.Ejikọtala mkpuchi karama ngwọta.
■ Iru mmiri na okpomọkụ nwere ike imetụta nsonaazụ.
∎ Mgbe usoro nyocha ahụ mechara, tụfuo swabs nke ọma mgbe ịmechara ya na 121ºC ma ọ dịkarịa ala nkeji iri abụọ.N'aka nke ọzọ, enwere ike ịgwọ ha na 0.5% sodium hypochloride (ma ọ bụ ịcha ọcha nke ụlọ) maka otu awa tupu atụfuo ya.Ekwesịrị ịtụfu ihe eji eme nnwale dịka iwu mpaghara, steeti na/ma ọ bụ gọọmenti etiti siri dị.
■ Ejila brushes cytology na ndị ọrịa dị ime.
Nchekwa na Nkwụsi ike
∎ Ekwesịrị idobe ngwa ahụ na 2-30C ruo mgbe ebibiela n'akpa mkpuchi mkpuchi.
■ Nnwale ahụ ga-adịrịrị n'ime obere akpa ahụ emechiri emechi ruo mgbe ejiri ya.
■ Ajụla oyi.
■ Ekwesịrị ịkpachapụ anya iji chebe ihe ndị dị na ngwa a pụọ na mmetọ.Ejila ma ọ bụrụ na enwere ihe akaebe nke mmetọ microbial ma ọ bụ mmiri ozuzo.Mmetọ ndu nke akụrụngwa na-ekesa, arịa ma ọ bụ reagents nwere ike ibute nsonaazụ ụgha.
Mkpokọta na ichekwa ihe atụ
Jiri naanị Dacron ma ọ bụ Rayon tipped swabs nwere ogwe rọba.A na-atụ aro ka iji swab nke onye na-emepụta kits na-enye (Swabs adịghị na ngwa a, maka ozi ịtụ, biko kpọtụrụ onye nrụpụta ma ọ bụ onye nkesa mpaghara, nọmba katalọgụ bụ 207000).Emebebeghị ka swabs sitere na ndị na-ebubata ngwaahịa ndị ọzọ.A naghị atụ aro swabs na ndụmọdụ owu ma ọ bụ osisi osisi.
■ A na-enweta ihe nlele site na iji swab polyester na-adịghị mma.Ekwesịrị ịnakọta ihe nlele ahụ tupu ịme nyocha dijitalụ na / ma ọ bụ ultrasound transvaginal.Kpachara anya ka ị ghara imetụ ihe ọ bụla aka na swab tupu ị were nlele ahụ.Jiri nlezianya tinye ọnụ ọnụ swab n'ime ikpu n'akụkụ fornix azụ ruo mgbe a zutere nguzogide.N'aka nke ọzọ, enwere ike iwere ihe nlele ahụ site na fornix azụ n'oge nyocha nke nlebara anya adịghị mma.Ekwesịrị ịhapụ swab n'ime ikpu maka 10-15 sekọnd iji mee ka ọ banye n'ime ihe nzuzo nke ikpu.Jiri nlezianya dọrọ swab ahụ!.
∎ Tinye swab n'ime tube mmịpụta, ọ bụrụ na enwere ike ịme ule ahụ ozugbo.Ọ bụrụ na ọ gaghị ekwe omume nnwale ozugbo, a ga-edobe ihe nlele onye ọrịa n'ime ọkpọkọ ebu njem kpọrọ nkụ maka nchekwa ma ọ bụ mbugharị.Enwere ike ịchekwa swabs ahụ maka awa 24 na ụlọ okpomọkụ (15-30 ° C) ma ọ bụ otu izu na 4 Celsius ma ọ bụ karịa ọnwa 6 na -20 Celsius.Ekwesịrị ikwe ka ụdịdị niile rute n'ime ụlọ nke 15-30ºC tupu nnwale.
Usoro
Weta ule, ụdị, ihe nchekwa na/ma ọ bụ njikwa n'ime ụlọ okpomọkụ (15-30°C) tupu eji ya.
∎ Tinye ọkpọkọ amịpụta dị ọcha n'ebe a họpụtara n'ebe a na-arụ ọrụ.Tinye 1ml nke ihe nchekwa mmịpụta na tube mmịpụta.
∎ Tinye swab n'ime tube.Gwakọta ihe ngwọta nke ọma site n'ịtụgharị swab ahụ n'ike n'akụkụ akụkụ tube ọ dịkarịa ala ugboro iri (mgbe mmiri na-emikpu).A na-enweta nsonaazụ kacha mma mgbe agwakọtara ihe atụ ahụ siri ike na ngwọta.
■ Wepụ mmiri mmiri dị ka o kwere mee site na swab site na ịpịa akụkụ nke tube mmịpụta na-agbanwe agbanwe ka ewepụrụ swab ahụ.Opekempe, 1/2 nke ihe nkpuchi ihe nlele ga-anọrịrị na tube maka ịkwaga capillary zuru oke ga-eme.Tinye okpu na tube ewepụtara.
Tụfuo swab ahụ n'ime akpa mkpofu ihe ize ndụ dị ndụ kwesịrị ekwesị.
∎ Ihe nlele ndị ewepụtara nwere ike idowe n'ime ụlọ okpomọkụ nkeji iri isii na-emetụtaghị nsonaazụ ule ahụ.
■ Wepụ ule ahụ na obere akpa ya mechiri emechi, ma tinye ya n'elu ala dị ọcha ma dịkwa larịị.Kpọọ ngwaọrụ ahụ na njirimara onye ọrịa ma ọ bụ njikwa.Iji nweta nsonaazụ kacha mma, a ga-eme nyocha ahụ n'ime otu awa.
∎ Tinye ntụsara ahụ 3 (ihe dị ka 100 µl) nke ihe nlele ewepụtara na tube Extraction na nlele nke ọma na cassette ule.
Zenarị ọnyà ikuku n'ụdị nke ọma (S), ma etinyekwala ngwọta ọ bụla na windo nlele.
Ka ule na-amalite ịrụ ọrụ, ị ga-ahụ ka agba na-aga n'ofe akpụkpọ ahụ.
∎ Chere ka eriri (s) nwere agba pụta.Ekwesịrị ịgụ nsonaazụ ya na nkeji ise.Atụgharịla nsonaazụ ya mgbe nkeji ise gachara.
Tụfuo ọkpọkọ ule ejiri mee ihe yana kasetị nwale n'ime akpa mkpofu ihe ize ndụ kwesịrị ekwesị.
NKWAKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWASỊA
| EZIOKWUNsonaazụ:
| Agbụ abụọ nwere agba na-apụta na akpụkpọ ahụ.Otu band na-egosi na mpaghara njikwa (C) na band ọzọ na-egosi na mpaghara ule (T). |
| NA-ADỊGHỊNsonaazụ:
| Naanị otu eriri agba na-apụta na mpaghara njikwa (C).Ọ nweghị eriri nwere agba pụtara na mpaghara ule (T). |
| abaghị uruNsonaazụ:
| Otu njikwa adaghị apụta.A ga-atụfurịrị nsonazụ sitere na ule ọ bụla nke na-emepụtabeghị igwe njikwa n'oge agụpụtara akọwapụtara.Biko lelee usoro a wee jiri ule ọhụrụ kwugharịa.Ọ bụrụ na nsogbu ahụ dịgidere, kwụsị iji ngwa ngwa ngwa wee kpọtụrụ onye na-ekesa mpaghara gị. |
IHE:
1. Ike nke agba na mpaghara ule (T) nwere ike ịdịgasị iche dabere na ntinye nke ihe eji eme ihe dị na ụdị.Ma bekee larịị-apụghị kpebisie ike a qualitative ule.
2. Oke ụdịdị ezughi oke, usoro arụ ọrụ ezighi ezi, ma ọ bụ ime ule emebiela bụ ihe kpatara ọdịda njikwa njikwa.
Njikwa ogo
∎ Agụnyere njikwa usoro ime n'ule ahụ.A na-ewere eriri agba na-apụta na mpaghara njikwa (C) dị ka njikwa usoro dị mma n'ime.Ọ na-akwado oke ihe atụ zuru oke yana usoro usoro ziri ezi.
∎ Enwere ike ịnye njikwa usoro mpụga (na arịrịọ naanị) n'ime ngwa ahụ iji hụ na ule na-arụ ọrụ nke ọma.Ọzọkwa, enwere ike iji njikwa ndị ahụ gosipụta arụmọrụ kwesịrị ekwesị site n'aka onye na-ahụ maka ule.Iji mee ule nchịkwa dị mma ma ọ bụ nke na-adịghị mma, mezue nzọụkwụ na ngalaba Usoro Nnwale na-emeso swab njikwa n'otu ụzọ ahụ dị ka swab ihe atụ.
Oke nke ule
1. Ọnweghị nkọwa ọnụọgụgụ ekwesịrị ịme dabere na nsonaazụ ule.
2.Do not eji ule ma ọ bụrụ na ya aluminum foil obere akpa ma ọ bụ akàrà nke obere akpa na-adịghị.
3.A nti StrongStep®Nsonaazụ ule PROM, n'agbanyeghị na ịchọpụta ọnụnọ mmiri amniotic dị na nlele ahụ, anaghị ahụ ebe mgbawa ahụ.
4.Dị ka ọ dị na nyocha nyocha niile, a ghaghị ịkọwa nsonaazụ ya na nyocha nke nchọpụta ụlọ ọgwụ ndị ọzọ.
5.Ọ bụrụ na mgbawa nke akpụkpọ anụ nwa ebu n'afọ emeela ma nkwụsị nke mmiri ọmụmụ akwụsịla ihe karịrị awa iri na abụọ tupu e were ihe atụ ahụ, IGFBP-1 nwere ike imebi site na proteases na ikpu na ule ahụ nwere ike inye nsonaazụ na-adịghị mma.
ÀJỤMỤ ỌRỤ
Tebụl: StrongStep®Ule PROM vs. Nnwale ika PROM ọzọ
|
Mmetụta dị n'etiti: |
| ika ọzọ |
| ||
| + | - | Mkpokọta | |||
| Nzọụkwụ siri ike®PROM Nwalee | + | 63 | 3 | 66 | |
| - | 2 | 138 | 140 | ||
|
| 65 | 141 | 206 | ||
Mmetụta nyocha
Ọnụ ọgụgụ kasị ala achọpụtara nke IGFBP-1 n'ime nlele amịpụtara bụ 12.5 μg/l.
Ihe na-egbochi
Ekwesịrị ịkpachapụ anya ka ị ghara iji mmanụ mmanu, ncha, ọgwụ nje, ma ọ bụ ude metọọ ngwa ngwa ma ọ bụ ihe nzuzo cervicovaginal.Ihe mmanu mmanu ma ọ bụ ude nwere ike igbochi nnabata nke ụdị n'ime ngwa.Ncha ma ọ bụ ihe na-egbu egbu nwere ike igbochi mmeghachi omume antibody-antigen.
A nwalere ihe ndị nwere ike itinye aka na mkpokọta nke enwere ike ịhụ na nzuzo nke cervicovaginal.Ihe ndị na-esonụ anaghị etinye aka na nyocha ahụ mgbe a na-anwale ya na ọkwa ndị egosiri.
| Ihe | Ntinye uche | Ihe | Ntinye uche |
| Ampicillin | 1.47 mg / ml | Prostaglandin F2 | 0.033 mg / ml |
| Erythromycin | 0.272 mg / ml | Prostaglandin E2 | 0.033 mg / ml |
| Urine nne nke atọ n'ime ọnwa atọ | 5% (vol) | MonistatR (miconazole) | 0.5 mg / ml |
| Oxytocin | 10 IU/ml | Indigo Carmine | 0.232 mg / ml |
| Terbutaline | 3.59 mg / ml | Gentamicin | 0.849 mg / ml |
| Dexamethasone | 2.50 mg / ml | Gel nke Betadine | 10 mg / ml |
| MgSO4•7H2O | 1.49 mg / ml | BetadineR Cleanser | 10 mg / ml |
| Ritodrine | 0.33 mg / ml | Jelii K-YR | 62.5 mg / ml |
| DermicidolR 2000 | 25.73 mg / ml |
Ntụaka agụmagụ
Erdemoglu na Mungan T. mkpa nke ịchọpụta insulin-dị ka ihe na-eto eto na-ejikọta protein-1 na nzuzo nzuzo nke cervicovaginal: atụnyere ule nitrazine na nyocha nke mmiri amniotic.Acta Obstet Gynecol Scand (2004) 83:622-626.
Kubota T na Takeuchi H. Nyocha nke insulin-dị ka ihe na-eto eto na-ejikọta protein-1 dị ka ngwá ọrụ nyocha maka mgbawa nke akpụkpọ ahụ.J Obstet Gynecol Res (1998) 24:411-417.
Rutanen EM et al.Nlele ule mgbawa ngwa ngwa maka insulin-dị ka ihe na-eto eto na-ejikọta protein-1 na nyocha nke akpụkpọ anụ nwa ebu n'afọ gbawara agbawa.Clin Chim Acta (1996) 253:91-101.
Rutanen EM, Pekonen F, Karkkainen T. Ntụle insulin-dị ka ihe na-eto eto na-ejikọta protein-1 na nzuzo nzuzo nke cervical/vaginal: atụnyere ROM-lelee Membrane Immunoassay na nyocha nke akpụkpọ anụ nwa ebu n'afọ gbawara agbawa.Clin Chim Acta (1993) 214:73-81 .
Nkọwa nkọwa
|
| Nọmba katalọgụ |  | Mmachi okpomọkụ |
 | Kpọtụrụ ntuziaka maka ojiji |
| Koodu ogbe |
 | In vitro diagnostic ngwaọrụ ahụike |  | Jiri ya |
 | Onye nrụpụta |  | Nwere ezuru makaule |
 | Ejikwala ya ọzọ |  | Onye nnọchi anya ikike na European Community |
 | CE akara dịka ntuziaka ngwaọrụ ahụike IVD 98/79/EC siri dị | ||
