Ule nnwale
Eji eji
Ike®Nnwale na-eme ka a tụgharịrị nyocha, nyocha immunoatographic maka nchọpụta igfbp-1 site na mmiri mmiri na-enweghị atụ n'oge ime. Ezubere ule ahụ maka iji ya mee ihe iji nyere aka chọpụta na mgbapu nke fatal membranes (rom) n'ime ụmụ nwanyị dị ime.
Ndubata
The ista nke Igfbp-1 (insulin-dị ka ihe dị ka protein-1) na mmiri mmiri dị ka 100000 dị elu karịa ka ọ dị. Igfbp-1 anaghị abụkarị na ikpu, mana mgbe itisịa Metbranes, mmiri mmiri na-enwe nnukwu iko nke Igfbp-1. Na nnwale nkwalite ike siri ike, a na-eji ụdị nzuzo nke polyester swab na-amịpụta mkpụrụ osisi na-enweghị atụ. Ọnụnọ Igfbp-1 na-achọpụta site na iji ngwaọrụ nnwale ngwa ngwa.
Prinsipul
Ike®Nnwale Mgbanwụ na-eji agba na-acha odo odo na-acha odo odo, nke Capillary Rich. Usoro ule chọrọ usoro nke Igfbp-1 site na swab swab site na ịgwakọta swab na Nchekwa nlele. Mgbe ahụ, a na-agbakwunye ihe nlereanya a gwakọtara ọnụ na-atụkwasị ihe nlele kassaste ule nke ọma na ngwakọta na-akwaga na akpụkpọ ahụ elu. Ọ bụrụ na Igfbp-1 dị na nlele ahụ, ọ ga-etolite mgbagwojuanya na mgbochi mgbochi 1 na-efe efe na-acha odo odo. Ihe ndị ahụ ga-eketa ya site na mgbochi nke abụọ nke na-emegide IGFBP-1 Antibodkpụrụ dị na akpụkpọ ahụ nitrocellose. Ọdịdị nke akara ule nnwale yana akara njikwa ga-egosi ezigbo nsonaazụ.
Kit mejupụtara
| 20 N'otu n'otu paceked nnwale ngwaọrụ | Ngwaọrụ ọ bụla nwere mpempe na-acha odo odo na ihe ndị na-eme ihe nkiri na-adịbu ọnụ na mpaghara kwekọrọ kwekọrọ. |
| 2MgwuchateVialf vial | 0.1 m phosphate a na-eji saline (PBS) na 0.02% sodium azide. |
| 1 ezigbo njikwa swab (na rịọrọ) | Nwere igfbp-1 na sodium azide. Maka nchịkwa mpụga. |
| A swab na-adịghị mma (na rịọrọ) | Enweghị igfbp-1. Maka nchịkwa mpụga. |
| 20 Mmịpụta akpa | Maka iji ngwa ahịa. |
| 1 Nke oru | Ebe maka ijide vials na tubes. |
| 1 Ngwa ngwugwu Tinye | Maka nkuzi ọrụ. |
Ihe achọrọ mana enyeghị ya
| Ihe ozo | Maka iji oge. |
MKPACHAPỤ ANYA
Maka iji naanị ya.
Ejila mgbe ụbọchị ngafe gosipụtara na ngwugwu. Ejila ule ahụ ma ọ bụrụ na obere akpa foot. Emela nyocha.
■ Ngwa a nwere ngwaahịa sitere na anụmanụ. Ihe omuma banyere mmalite nke sitere na / ma ọ bụ ịdị ọcha nke ụmụ anụmanụ anaghị ekwe nkwa na enweghị ndị nnọchi anya nje. Ọ bụ ya mere, ka akwadoro na a na-emeso ngwaahịa ndị a ka enwere ike na-efe efe, ma na-elezi anya na-akpachapụ anya nchekwa nchekwa (enweghị ume ma ọ bụ iku ume).
Zere ihe mgbochi nke ọdịnala site na iji akpa nchịkọta ọhụụ maka ụdị ọ bụla nwetara.
Gụgharịa usoro ahụ niile na-akpachapụ anya tupu ịme nnwale ọ bụla.
Erila nri, ị drinkụ mmanya ma ọ bụ anwụrụ ọkụ na mpaghara ebe a na-edozi ụdị osisi na Kits. Jirikwa ụdị niile dị ka a ga-asị na ha nwere ndị ọrụ na-efe efe. Legodị ihe ndị a na-eme atụmatụ na-emegide ihe ọghọm na usoro microbiological niile ma soro usoro ọkọlọtọ maka ụdị nke ụdị. Yiri uwe nchebe dị ka mkpuchi ụlọ nyocha, a na-ewepụ akwa na nchedo anya mgbe a na-ekwu okwu.
Etinyekwala nsogbu ma ọ bụ na-agwakọta akwụkwọ ozi dị iche iche. Ejikọtala mkpụrụ osisi karama.
■ iru mmiri na ọnọdụ okpomọkụ nwere ike imetụta nsonaazụ.
■ Mgbe emechara usoro doro anya, gbanye swabs nke ọma mgbe autocling ha na 121 Celsius C ruo opekata mpe nkeji iri abụọ. N'aka nke ọzọ, enwere ike ịgwọ ha na 0,5% sodium hypochloride (ma ọ bụ na-ejide ya n'ụlọ) maka otu awa tupu otu awa tupu otu elekere. A ga-atụfu ihe eji ewu ewu na mpaghara, steeti na / ma ọ bụ iwu gọọmentị.
■ Ejila ahịhịa na-arịa ọrịa cytology.
Nchekwa na nkwụsi ike
A ga-echekwa kit a na 2-30 Celsius C ruo ụbọchị ebiputere ya na akpa akara.
■ Ule a ga-anọgide na akpaghị aka ma ọ bụrụ na ojiji.
■ Ejila ihe.
■ A ga-eme ka a ga-akpachara anya iji chebe ihe ndị a na-eme. Ejila ma ọ bụrụ na enwere ihe akaebe nke nje ikuku ma ọ bụ ọdịda. Mmetọ dị ndụ nke inyefe akụ, ndị na-eme ihe ma ọ bụ ndị na-eme ihe ga-eduga na nsonaazụ ụgha.
Nchịkọta ụdị na nchekwa
Jiri naanị dacron ma ọ bụ rayon tisabs na-enweghị atụ. Ọ na-atụ aro iji swab nyere swab e nyere swab nyere (swabs a, maka ozi a na-eme ihe, biko kpọtụrụ onye na-emepụta obodo, ọnụ ọgụgụ Cataloge bụ 207000). A naghị ahazi swabs site na ndị ọzọ na-ebubata ndị ọzọ. A naghị atụ aro ka swabs na akwa ma ọ bụ osisi osisi.
A na-enweta ihe nlere iji na-eji swab na-atụ mmiri polyester. A ga-anakọta ihe atụ ahụ tupu ịme nyocha dijitalụ na / ma ọ bụ ultraginal ultraginal. Kpachara anya ka ị ghara imetụ swab aka tupu ịlele ihe nlele ahụ. Jiri nwayọ tinye onu nke swab n'ime polina n'ebe onye na-ahụ maka postnix ruo mgbe ezute nguzogide. N'aka nke ọzọ, a nwere ike iwere ihe nlele ahụ site na postnix postnix n'oge nyocha na-enweghị atụ. A ga-ahapụ swab na-apụ apụ na sekọnd 10-15 iji nye ya ohere ịmịkọrọ senlinal. Wepụ swab nke ọma!.
Tinye a mmịpụta ahụ na mmịpụta ahụ, ọ bụrụ na ule ahụ nwere ike na-agba ọsọ ozugbo. Ọ bụrụ na nnwale ozugbo agaghị ekwe omume, a ga-etinye ihe atụ onye ọrịa ahụ na tube akọrọ maka nchekwa ma ọ bụ ụgbọ. Enwere ike ịchekwa swabs maka awa 24 na ụlọ okpomọkụ (15-30 Celsius C) ma ọ bụ izu 1 na 4 Celsius C ma ọ bụ karịa ọnwa 6 na -20 Celsius C. A ga-ahapụ ụdị niile ị ga-eji rute ụlọ nke 15-30 ° C tupu ule.
Uzo esi eme ihe
Weta ule, ọdịdị, atụmatụ na / ma ọ bụ njikwa ụlọ okpomọkụ (15-30 Celsius C) tupu ojiji.
Debe ndọtị dị ọcha na mpaghara ahaziri. Tinye 1ML nke mmịpụta na mmịpụta na mmịpụta.
Debe ụdị ahụ swab na tube. Jiri nwayọ gwakọta ihe ngwọta site na ịmegharị swab ike megide akụkụ nke tube ahụ ruo opekata mpe iri (ebe ọ na-emebi). A na - enweta nsonaazụ kachasị mma mgbe ụdị ahụ na - agbakọta na ngwọta ya.
Dọpụsịla mmiri mmiri dị ka o kwere mee site na paob site na iji ya na-akụda akụkụ nke mmịpụta na-agbanwe agbanwe ka a na-ewepụ swab. Dịkarịa ala, 1/2 nke ihe nlele nlele ga-anọgide na tube maka ọpụpụ caparillary ga-eme. Tinye okpu na tube wepụtara.
Tụfuo swab na nnukwu ihe mkpofu biohaardous.
Thistumens ndị ahụ wepụtara nwere ike idobe na ụlọ okpomọkụ ruo minit 60 na-emetụtaghị nsonaazụ.
Wepu ule ahụ site na obere akpa ya akara, ma tinye ya n'elu elu dị ọcha, ọkwa dị larịị. Jiri onye ọrịa kpọọ ngwaọrụ ma ọ bụ ịchọpụta. Iji nweta nsonaazụ kachasị mma, ekwesịrị ịme ya n'ime otu elekere.
Tinye 3 tụlee (ihe dị ka 100 μl) nke ihe nlele sitere na mmịpụta nke ọma na sample ahụ nke ọma na cassette ule.
Zere ọnya ikuku na -eche na ntụsara ahụ nke ọma (s), ma ọ gaghị etinye ihe ngwọta ọ bụla na windo nlele.
Dịka ule ahụ malitere ịrụ ọrụ, ị ga-ahụ na agba na-agafere akpụkpọ ahụ.
■ Chere maka ndị nwere agba (s) na-egosi. Nsonaazụ a ga-agụ na nkeji ise. Emetụla nsonaazụ ya mgbe 5 nkeji.
Tụfuo tubes ule na cassettes na-eme ihe mkpofu ahịhịa dị mma.
Nbuputa nke nsonaazụ
| Nke omaRizootu:
| A na-eji akpụkpọ osisi abụọ na-apụta na akpụkpọ ahụ. Otu otu na-egosi na mpaghara nchịkwa (c) na otu ọzọ na-egosi na mpaghara nnwale (t). |
| NjuRizootu:
| Naanị otu otu nwere agba na-egosi na mpaghara njikwa (c). Enweghị akara nwere agba siri ike na-apụta na mpaghara nnwale (t). |
| AsiRizootu:
| Njikwa mmachi ada ada. Na-esite na nnwale ọ bụla nke na-emebeghị mmachi akara na oge ọgụgụ akọwapụtara na oge a ga-agịga. Biko gụgharịa usoro ma na-emeghachi ya na nnwale ọhụrụ. Ọ bụrụ na nsogbu ahụ dịgidere, kwụsịtụrụ iji Kit ozugbo ma kpọtụrụ onye na-ekesa gị ihe. |
Mara:
1. Oke nke agba na mpaghara nnwale (t) nwere ike ịdị iche na ntinye uche nke ihe ndị dị na ya. Ma ule ihe ndị dị na ya enweghị ike ikpebi.
2. Okpu olu ezughi oke, usoro arụ ọrụ na-ezighi ezi, ma ọ bụ na-arụ ọrụ e siri ike bụ ihe kachasị mma maka njikwa mmachi.
Njikwa Dị Mma
Agụnyere njikwa usoro nke ime na ule ahụ. A na-ahụta otu egwuregwu na-acha odo odo na mpaghara njikwa (C) ka a na-ewere dị ka usoro nchịkwa dị mma. Ọ na-egosi oke ụda olu zuru ezu na usoro usoro ziri ezi.
■ Ihe njikwa usoro nwere ike inye (na arịrịọ naanị) na kits iji hụ na ule ahụ na-arụ ọrụ nke ọma. Ọzọkwa, njikwa ndị ahụ nwere ike igosipụta arụmọrụ kwesịrị ekwesị site na onye ọrụ nnwale. Iji mee nyocha dị mma ma ọ bụ nke na-adịghị mma, mezue usoro na ngalaba nnwale na-emeso swab na-elezi anya n'otu ụzọ ahụ dị ka a swab swab.
Ike ule
1. Ekwesighi iji ya mee ihe kwesiri ekwesi na Nsonaazụ ule.
2.JEGHARA na-eji ule ahụ ma ọ bụrụ na akpa osisi a aluminom ya ma ọ bụ akara nke akpa ahụ agaghị emebi.
3.A kwesịrị ekwesị®Nsonaazụ nnwale, n'agbanyeghi na ịchọpụta ọnụnọ nke mmiri amniotic na ihe nlele ahụ, ọ dịghị ebe a na-achọ saịtị nke mgbapu.
4.Am nwere ule nyocha niile, a ga-asughari ya na ihe nchoputa nke ulo ogwu ndi ozo.
Metbụ metbranes nke Ftalbranes emeela mana a na-ebugharị mmiri mmiri na-agabiga ihe karịrị awa iri na abụọ tupu igfbp-1 nwere ike ibute nsonaazụ na-adịghị mma.
Njirimara arụmọrụ
Tebụl: Echiche®Nnwale nnwale vs. ọzọ nnwale
|
Mmetụta mmetụta dị iche iche: |
| Akara ozo |
| ||
| + | - | Kemgbakota | |||
| Ike®Nkwa Inwa | + | 63 | 3 | 66 | |
| - | 2 | 138 | 6.0 | ||
|
| 65 | 141 | 206 | ||
Nchọpụta nyocha
Onu ogugu nke Igfbp-1 na sample ewepụtara bụ 12.5 μg / l.
Na-egbochi ihe
A ga-enwerịrị nlekọta agaghị emerụ onye na-etinye akwụkwọ ma ọ bụ cervicvavagine na-akpụ akpụ na lubricants, ncha, ịmị mkpụrụ, ma ọ bụ ude. Lubricants ma ọ bụ ude nwere ike igbochi na ịmịcha ahụ na onye na-etinye ya. Ncha ma ọ bụ ọgwụ mgbochi nwere ike igbochi mmeghachi omume antibun.
A nwalere ihe ndị nwere ike igbochi ihe ndị nwere ike ịchọta ya na ngalaba cervicovaginal. Ihe ndị a na-esote emeghị ka ihe dị na arụ ọrụ mgbe a nwalere na ọkwa egosipụtara.
| Umi | Ntinye echiche | Umi | Ntinye echiche |
| Ampilillin | 1.47 mg / ml | Prostaglan f2 | 0.033 mg / ml |
| Erythomycin | 0.272 mg / ml | Prostaglan E2 | 0.033 mg / ml |
| Mmamiri na-eme egwuregwu | 5% (Vol) | Monistatr (micnetzole) | 0,5 mg / ml |
| Oxytocin | 10 iU / ml | Indigo carmine | 0.232 mg / ml |
| Terbutaline | 3.59 mg / ml | Redinamicin | 0.849 mg / ml |
| Dexamethasone | 2.50 mg / ml | Betadiner gel | 10 mg / ml |
| Mgso4•7.H2O | 1.49 mg / ml | Betadiner dị ọcha | 10 mg / ml |
| Ritrine | 0.33 mg / ml | K-YR jelii | 62.5 mg / ml |
| Dermicidolr 2000 | 25.73 mg / ml |
Ntụgharị akwụkwọ
Erdemoglu na mungan T. Mkpa ịchọpụta insulin-dị ka ihe na-eme ka protein-1 na nzuzo cernicovaginal nyocha na nyocha olu dị egwu. Isam obstet Gynocol Scand (2004) 83: 622-626.
Kubota t na sauchi H. Nyocha nke insulin-dị ka ihe dị ka ihe protein-1 dị ka ngwa ọrụ maka mgbapu nke membranes. Jybstet gynecol res (1998) 24: 411-417.
Rutanen m et al. Ntụle nke nnwale dị ngwa maka insulin-dị ka ihe na-achọ protein-1 na nchọta nke membranes felbranes. GLỌ CHIM Chim Aca (1996) 253: 91-101.
Rutanen em, Pekonen f, karkainen t. Nile nke insulin-dị ka ihe na-eme ka protein-1 na-ekiri ihe nkiri na-enyocha flay-lele nke membranes. Glọnlọn chim na-eme ihe (1993) 214: 73-81.
Egosipụta akara
|
| Nọmba katalọgụ |  | Mmachi okpomọkụ |
 | Pụọ ntuziaka maka iji |
| Koodu bat |
 | Na vitronic Victostic ngwaọrụ |  | Jiri |
 | Mmewe |  | Nwere nke zuru ezu maka |
 | Etula ya |  | Onye nnọchite anya ikike na European Community |
 | Akara dị ka ngwaọrụ ọgwụ Ivd Ivd dị na ya na-eduzi 98/79 / EE | ||
