Anyị nwere ọtụtụ ule nyocha dị.
Chọpụta ihe gbasara ule ọ bụla na ngwugwu ngwaahịa anyị n'okpuru.
Akwụkwọ akụkọ Coronavirus (SARS-CoV-2) Ngwa PCR Real-Time Multiplex
Igwe q PCR kwesịrị iru ihe ndị a chọrọ:
1. dabara 8 warara PCR tube olu 0.2 ml
2. Nwee ọwa nchọpụta ihe karịrị anọ:
Ọwa | Mmasị (nm) | Mgbapụta (nm) | Ejiji agbachagoro tupu oge eruo |
1. | 470 | 525 | FAM, SYBR Green I |
2 | 523 | 564 | VIC, HEX, TET, JOE |
3. | 571 | 621 | ROX, TEXAS-RED |
4 | 630 | 670 | CY5 |
PCR-ikpo okwu:
Sistemụ PCR 7500 Real-Time, Biorad CF96, iCycler iQ™ Sistemụ Nchọpụta PCR Real-Time, Stratagene Mx3000P, Mx3005P