Ule FETAL FIBRECON ngwa ngwa
Na-eji
Ike®Ebumnuche na-eme ka a tụgharịrị nyocha immunoatographic ule immunoatographic nke achọrọ maka nchọpụta ruru eru maka nzuzo ceticovaginal. Ọnụnọ nke fetal Fibronorlin na nzuzo cervicovaginal na nzuzo, ụbọchị 0 na izu ụka 34, ụbọchị 64 gacharametụtara ihe dị elu n'ihe ize ndụ nke nnyefe nke onye na-ebugharị.
Trosetional
Ibufe onye na - ebugharị ya, nke Aclecy College nke America kọwara, nke Acle Collegetricias na Gynecomiless na-enyefe tupu izu nke iri atọ na asaa nke ihe na-abụghị chromosimal perbination na-enweghị ọrịa. Ihe mgbaàmà nke nnyefe onye na - enye onye na - enye ya egwu, gbanwee nke ndọpụ uterine, ọbara ọgbụgba, ọghọm afọ, na ihe mgbochi. Ọnọdụ mgbanwe maka njirimara nke nnyefe nke onye na-atụnye ụtụ na-etinye akparamagwa nke Uterine ọrụ nyocha na arụmọrụ nke nyocha dijitalụ, nke na-ahapụ atụmatụ cervical akụkụ. E gosipụtawo ụzọ ndị a, dị ka obere cervical dị mma (<3 centimeter) na ọrụ isterine na-eme ọfụma ma bụrụchaghị nyocha nke nnyefe nke onye na-ebugharị. Ọ bụ ezie na enyochala ihe ndị dị mkpa akara akara, ọ dịghị onye ọ bụla nabatara nke ọma maka ọrụ ụlọ ọgwụ.
FETAL FIBRONRIN (FFN), acoform nke fibroonecin, bụ ihe dị mgbagwoju anya Glycoprotein nwere oke molecular nke ihe dị ka 500,000. Matsuura na ndị na-elekọta ndị ọrụ akọwarala a na-akpọ FDC-6, nke ghotara III-CS, mpaghara ahụ na-akọwa nwa ebu n'afọ nke fibrorm. Ọmụmụ Immunohstemical nke Plasentae egosila na FFN bụjikọtara ya na matriks nke mpaghara na-akọwapụta okwu ahụnke nne na nwa ebu n'afọ n'ime akpanwa.
Enwere ike ịchọpụta fiblorectin nwere ike ịchọpụta na nzuzo nke ụmụ nwanyị carvicovaginal na ime ime mmiri immunodbasy. Fetal fibronectin is elevated in cervicovaginal secretions during early pregnancy but is diminished from 22 to 35 weeks in normal pregnancies. Aghọtaghị ihe ọnụnọ ọnụ dị na nwanyị n'oge izu ime ime aghọtaghị. Agbanyeghị, ọ nwere ike gosipụta uto dị mma nke ọnụ ọgụgụ Trovoplast na Plasenta. A na-akọ banyere FFN na nzuzo cervicovaginal n'etiti izu 22, a na-akọ na ọ ga-eme ka ụbọchị iri abụọ na anọ, ụbọchị iri isii na iri atọ na Assttoptomatic Willed.
Prinsipul
Ike®Nnwale FFN na-eji agba eme ọgwụ mgbochi immulary, nke Capillary Rich. Usoro nnwale chọrọ ka ọ bụrụ ffn site na swab swab site na ịgwakọta swab na Nchekwa nlele. Mgbe ahụ, a na-agbakwunye ihe nlereanya a gwakọtara ọnụ na-atụkwasị ihe nlele kassaste ule nke ọma na ngwakọta na-akwaga na akpụkpọ ahụ elu. Ọ bụrụ na FFN nọ na nlele ahụ, ọ ga-etolite mgbagwojuanya na mgbochi mgbochi FFN na-egbochi ndị na-acha odo odo. Ihe mgbochi ahụ ga - abụ onye na - egbochi nke abụọ nke na - egbochi nke abụọ e ji akpụkpọ anụ nitrocellose. Ọdịdị nke akara ule nnwale yana akara njikwa ga-egosi ezigbo nsonaazụ.
Kit mejupụtara
| 20 N'otu n'otu paceked nnwale ngwaọrụ | Ngwaọrụ ọ bụla nwere mpempe na-acha odo odo na ihe ndị na-eme ihe nkiri na-adịbu ọnụ na mpaghara kwekọrọ kwekọrọ. |
| 2MgwuchateVialf vial | 0.1 m phosphate a na-eji saline (PBS) na 0.02% sodium azide. |
| 1 ezigbo njikwa swab (na rịọrọ) | Nwere ffn na sodium azide. Maka nchịkwa mpụga. |
| A swab na-adịghị mma (na rịọrọ) | Enweghị ffn. Maka nchịkwa mpụga. |
| 20 Mmịpụta akpa | Maka iji ngwa ahịa. |
| 1 Nke oru | Ebe maka ijide vials na tubes. |
| 1 Ngwa ngwugwu Tinye | Maka nkuzi ọrụ. |
Ihe achọrọ mana enyeghị ya
| Ihe ozo | Maka iji oge. |
MKPACHAPỤ ANYA
Maka iji naanị ya.
Ejila mgbe ụbọchị ngafe gosipụtara na ngwugwu. Ejila ule ahụ ma ọ bụrụ na obere akpa foot. Emela nyocha.
■ Ngwa a nwere ngwaahịa sitere na anụmanụ. Ihe omuma banyere mmalite nke sitere na / ma ọ bụ ịdị ọcha nke ụmụ anụmanụ anaghị ekwe nkwa na enweghị ndị nnọchi anya nje. Ọ bụ ya mere, ka akwadoro na a na-emeso ngwaahịa ndị a ka enwere ike na-efe efe, ma na-elezi anya na-akpachapụ anya nchekwa nchekwa (enweghị ume ma ọ bụ iku ume).
Zere ihe mgbochi nke ọdịnala site na iji akpa nchịkọta ọhụụ maka ụdị ọ bụla nwetara.
Gụgharịa usoro ahụ niile na-akpachapụ anya tupu ịme nnwale ọ bụla.
Erila nri, ị drinkụ mmanya ma ọ bụ anwụrụ ọkụ na mpaghara ebe a na-edozi ụdị osisi na Kits. Jirikwa ụdị niile dị ka a ga-asị na ha nwere ndị ọrụ na-efe efe. Legodị ihe ndị a na-eme atụmatụ na-emegide ihe ọghọm na usoro microbiological niile ma soro usoro ọkọlọtọ maka ụdị nke ụdị. Yiri uwe nchebe dị ka mkpuchi ụlọ nyocha, a na-ewepụ akwa na nchedo anya mgbe a na-ekwu okwu.
Etinyekwala nsogbu ma ọ bụ na-agwakọta akwụkwọ ozi dị iche iche. Ejikọtala mkpụrụ osisi karama.
■ iru mmiri na ọnọdụ okpomọkụ nwere ike imetụta nsonaazụ.
■ Mgbe emechara usoro doro anya, gbanye swabs nke ọma mgbe autocling ha na 121 Celsius C ruo opekata mpe nkeji iri abụọ. N'aka nke ọzọ, enwere ike ịgwọ ha na 0,5% sodium hypochloride (ma ọ bụ na-ejide ya n'ụlọ) maka otu awa tupu otu awa tupu otu elekere. A ga-atụfu ihe eji ewu ewu na mpaghara, steeti na / ma ọ bụ iwu gọọmentị.
■ Ejila ahịhịa na-arịa ọrịa cytology.
Nchekwa na nkwụsi ike
A ga-echekwa kit a na 2-30 Celsius C ruo ụbọchị ebiputere ya na akpa akara.
■ Ule a ga-anọgide na akpaghị aka ma ọ bụrụ na ojiji.
■ Ejila ihe.
■ A ga-eme ka a ga-akpachara anya iji chebe ihe ndị a na-eme. Ejila ma ọ bụrụ na enwere ihe akaebe nke nje ikuku ma ọ bụ ọdịda. Mmetọ dị ndụ nke inyefe akụ, ndị na-eme ihe ma ọ bụ ndị na-eme ihe ga-eduga na nsonaazụ ụgha.
Nchịkọta Pecumen na nchekwa
■ Jiri naanị Dacron ma ọ bụ rayon tisabs na-enweghị atụ. Ọ na-atụ aro iji swab nyere swab e nyere swab nyere (swabs a, maka ozi a na-eme ihe, biko kpọtụrụ onye na-emepụta obodo, ọnụ ọgụgụ Cataloge bụ 207000). A naghị ahazi swabs site na ndị ọzọ na-ebubata ndị ọzọ. A naghị atụ aro ka swabs na akwa ma ọ bụ osisi osisi.
A na - enweta nzuzo crvictovaginal site na polyterior fornix nke ikpu. Emere usoro nchịkọta ahụ ka ọ dị nwayọ. Achọghị nchịkọta ma ọ bụ nchịkọta ike, ndị nkịtị maka microbiological ọdịnala, achọghị. N'oge nyocha a na-enyocha ya, tupu nyocha ọ bụla ma ọ bụ ọdịdị nke cervix ma ọ bụ nke na-adọba ụgbọala na-eme ka ọ dị ihe dị ka sekọnd iri iji nwee ike cervic. Agbalị ịnwale iji saterate onye na-etinye akwụkwọ nwere ike imebi ule ahụ. Wepu onye na-etinye akwụkwọ ma rụọ ọrụ ahụ dị ka enyere gị n'okpuru.
Tinye a mmịpụta ahụ na mmịpụta ahụ, ọ bụrụ na ule ahụ nwere ike na-agba ọsọ ozugbo. Ọ bụrụ na nnwale ozugbo agaghị ekwe omume, a ga-etinye ihe atụ onye ọrịa ahụ na tube akọrọ maka nchekwa ma ọ bụ ụgbọ. Enwere ike ịchekwa swabs maka awa 24 na ụlọ okpomọkụ (15-30 Celsius C) ma ọ bụ izu 1 na 4 Celsius C ma ọ bụ karịa ọnwa 6 na -20 Celsius C. A ga-ahapụ ụdị niile ị ga-eji rute ụlọ nke 15-30 ° C tupu ule.
Uzo esi eme ihe
Weta ule, ọdịdị, atụmatụ na / ma ọ bụ njikwa ụlọ okpomọkụ (15-30 Celsius C) tupu ojiji.
Debe ndọtị dị ọcha na mpaghara ahaziri. Tinye 1ML nke mmịpụta na mmịpụta na mmịpụta.
Debe ụdị ahụ swab na tube. Jiri nwayọ gwakọta ihe ngwọta site na ịmegharị swab ike megide akụkụ nke tube ahụ ruo opekata mpe iri (ebe ọ na-emebi). A na - enweta nsonaazụ kachasị mma mgbe ụdị ahụ na - agbakọta na ngwọta ya.
Dọpụsịla mmiri mmiri dị ka o kwere mee site na paob site na iji ya na-akụda akụkụ nke mmịpụta na-agbanwe agbanwe ka a na-ewepụ swab. Dịkarịa ala, 1/2 nke ihe nlele nlele ga-anọgide na tube maka ọpụpụ caparillary ga-eme. Tinye okpu na tube wepụtara.
Tụfuo swab na nnukwu ihe mkpofu biohaardous.
Thistumens ndị ahụ wepụtara nwere ike idobe na ụlọ okpomọkụ ruo minit 60 na-emetụtaghị nsonaazụ.
Wepu ule ahụ site na obere akpa ya akara, ma tinye ya n'elu elu dị ọcha, ọkwa dị larịị. Jiri onye ọrịa kpọọ ngwaọrụ ma ọ bụ ịchọpụta. Iji nweta nsonaazụ kachasị mma, ekwesịrị ịme ya n'ime otu elekere.
Tinye 3 tụlee (ihe dị ka 100 μl) nke ihe nlele sitere na mmịpụta nke ọma na sample ahụ nke ọma na cassette ule.
Zere ọnya ikuku na -eche na ntụsara ahụ nke ọma (s), ma ọ gaghị etinye ihe ngwọta ọ bụla na windo nlele.
Dịka ule ahụ malitere ịrụ ọrụ, ị ga-ahụ na agba na-agafere akpụkpọ ahụ.
■ Chere maka ndị nwere agba (s) na-egosi. Nsonaazụ a ga-agụ na nkeji ise. Emetụla nsonaazụ ya mgbe 5 nkeji.
Tụfuo tubes ule na cassettes na-eme ihe mkpofu ahịhịa dị mma.
Nbuputa nke nsonaazụ
| Nke omaRizootu:
| A na-eji akpụkpọ osisi abụọ na-apụta na akpụkpọ ahụ. Otu otu na-egosi na mpaghara nchịkwa (c) na otu ọzọ na-egosi na mpaghara nnwale (t). |
| NjuRizootu:
| Naanị otu otu nwere agba na-egosi na mpaghara njikwa (c). Enweghị akara nwere agba siri ike na-apụta na mpaghara nnwale (t). |
| AsiRizootu:
| Njikwa mmachi ada ada. Na-esite na nnwale ọ bụla nke na-emebeghị mmachi akara na oge ọgụgụ akọwapụtara na oge a ga-agịga. Biko gụgharịa usoro ma na-emeghachi ya na nnwale ọhụrụ. Ọ bụrụ na nsogbu ahụ dịgidere, kwụsịtụrụ iji Kit ozugbo ma kpọtụrụ onye na-ekesa gị ihe. |
Mara:
1. Oke nke agba na mpaghara nnwale (t) nwere ike ịdị iche na ntinye uche nke ihe ndị dị na ya. Ma ule ihe ndị dị na ya enweghị ike ikpebi.
2. Okpu olu ezughi oke, usoro arụ ọrụ na-ezighi ezi, ma ọ bụ na-arụ ọrụ e siri ike bụ ihe kachasị mma maka njikwa mmachi.
Njikwa Dị Mma
Agụnyere njikwa usoro nke ime na ule ahụ. A na-ahụta otu egwuregwu na-acha odo odo na mpaghara njikwa (C) ka a na-ewere dị ka usoro nchịkwa dị mma. Ọ na-egosi oke ụda olu zuru ezu na usoro usoro ziri ezi.
■ Ihe njikwa usoro nwere ike inye (na arịrịọ naanị) na kits iji hụ na ule ahụ na-arụ ọrụ nke ọma. Ọzọkwa, njikwa ndị ahụ nwere ike igosipụta arụmọrụ kwesịrị ekwesị site na onye ọrụ nnwale. Iji mee nyocha dị mma ma ọ bụ nke na-adịghị mma, mezue usoro na ngalaba nnwale na-emeso swab na-elezi anya n'otu ụzọ ahụ dị ka a swab swab.
Ike ule
1. Enwere ike iji ego a mee ihe maka ịchọpụta ogo nke fetal Fibronnortin na mpaghara cervicovaginal.
2. A ga-eji usoro nnwale kwesịrị iji ya na njikọ ụlọ ọgwụ ndị ọzọ na ụlọ nyocha maka njikwa onye ọrịa.
3. A ga-enweta ụdị ụdị tupu nyocha ma ọ bụ nyocha nke cervix. Nchịkọta nke Cervix nwere ike ibute nsonaazụ ụgha.
4. Ekwesịrị ịnakọta ụdị ma ọ bụrụ na onye ọrịa enweela mmekọahụ n'ime awa 24 iji kpochapụ nsonaazụ dị mma ụgha.
5. Ndị ọrịa nwere ndị ọrịa nwere ndị na-enyo enyo ma ọ bụ nke a na-ama ama, ma ọ bụ Plasenta na-agbanwe agbanwe ma ọ bụ nke na-enweghị ike ịnwale.
6. Agaghị anwale ndị ọrịa.
7. Njirimara arụmọrụ nke ike®Nnwale FFN sitere na ụmụ nwanyị nwere ihe ndị na-egbu egbu. Ekwela ka arụpụta arụmọrụ na ndị ọrịa nwere ọtụtụ ebe ọ bụla, dịka, ejima.
8. Ndị siri ike®Achọghị ule FFN na ọnụnọ nke imekpọmbranes ambimes na mgbapu nke emembranes ambimes ambranes a ga-achị tupu ha emee ule ahụ.
Njirimara arụmọrụ
Tebụl: Nnwale Elu FFN FFN
| Mmetụta mmetụta dị iche iche: 97.96% (89.13% -99.95%) * Nkọwapụta nke ikwu: 98.73% (95.50% -99.85%) * Nkwekọrịta na nkwekọrịta: 98.55% (95.82% -99.70%) * * 95% ntụkwasị obi |
| Akara ozo |
| ||
| + | - | Kemgbakota | |||
| Ike®fFn Inwa | + | 48 | 2 | 50 | |
| - | 1 | 156 | 157 | ||
|
| 49 | 158 | 207 | ||
Nchọpụta nyocha
Ọnụ ego kachasị na FFN na nlele ewepụtara bụ 50μG / l.
N'etiti ụmụ nwanyị sara mbara, ọkwa dị elu (≥ 0.050 μg / ml) (ụbọchị iri na atọ, ụbọchị iri na anọ ma ọ bụ ụbọchị 14 site na nchịkọta nlele. N'ime ụmụ nwanyị Asymptomatic, ọkwa dị elu nke FFN dị n'etiti izu 22, ụbọchị iri atọ, ụbọchị 6 na-egosi mmụba na izu iri atọ na atọ, ụbọchị mbido. Esemokwu nke 50 μg / l FFN ka a na-eduzi ọmụmụ ihe dị iche iche iji nyochaa mkpakọrịta dị n'etiti fatalinin ngosipụta n'oge ime na onye na-ebu ụzọ.
Na-egbochi ihe
A ga-enwerịrị nlekọta agaghị emerụ onye na-etinye akwụkwọ ma ọ bụ cervicvavagine na-akpụ akpụ na lubricants, ncha, ịmị mkpụrụ, ma ọ bụ ude. Lubricants ma ọ bụ ude nwere ike igbochi na ịmịcha ahụ na onye na-etinye ya. Ncha ma ọ bụ ọgwụ mgbochi nwere ike igbochi mmeghachi omume antibun.
A nwalere ihe ndị nwere ike igbochi ihe ndị nwere ike ịchọta ya na ngalaba cervicovaginal. Ihe ndị a na-esote emeghị ka ihe dị na arụ ọrụ mgbe a nwalere na ọkwa egosipụtara.
| Umi | Ntinye echiche | Umi | Ntinye echiche |
| Ampilillin | 1.47 mg / ml | Prostaglan f2 | a0.033 mg / ml |
| Erythomycin | 0.272 mg / ml | Prostaglan E2 | 0.033 mg / ml |
| Mmamiri na-eme egwuregwu | 5% (Vol) | Monistatr (micnetzole) | 0,5 mg / ml |
| Oxytocin | 10 iU / ml | Indigo carmine | 0.232 mg / ml |
| Terbutaline | 3.59 mg / ml | Redinamicin | 0.849 mg / ml |
| Dexamethasone | 2.50 mg / ml | Betadiner gel | 10 mg / ml |
| Mgso4•7.H2O | 1.49 mg / ml | Betadiner dị ọcha | 10 mg / ml |
| Ritrine | 0.33 mg / ml | K-YR jelii | 62.5 mg / ml |
| Dermicidolr 2000 | 25.73 mg / ml |
Ntụgharị akwụkwọ
1 Na-arụsi ọrụ ike. Mpempe akwụkwọaka, Nọmba 133, Ọktọba, 1989.
2. Crearey Rk, Recnick R. nne nwa na fecal: ụkpụrụ na omume. Filadelphia: WB Suunders; 1989.
3. Crearey Rk, Merkatz IR. Mgbochi ọmụmụ nke onye na-agba mmiri: echiche ụlọ ọgwụ. Obstet Gynecol 1990; 76 (nke 1): 2S-4s.
4. Morrison Jc. Nwa nwoke na-ebu ụzọ: ihe omimi dị mma idozi. Obstet Gynecol 1990; 76 (nke 1): 5s-12s.
5. Mkpọchi CJ, Senyei A, Disch Mr, Canal DC, et al. FETAL FIBRECECIN na CVICICE NA EGO NA-EGO EGO. New Eng J Med 1991; 325: 669-74.
Egosipụta akara
|
| Nọmba katalọgụ |  | Mmachi okpomọkụ |
 | Pụọ ntuziaka maka iji |
| Koodu bat |
 | Na vitronic Victostic ngwaọrụ |  | Jiri |
 | Mmewe |  | Nwere nke zuru ezu maka |
 | Etula ya |  | Onye nnọchite anya ikike na European Community |
 | Akara dị ka ngwaọrụ ọgwụ Ivd Ivd dị na ya na-eduzi 98/79 / EE | ||
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Ngwaọrụ FIBSTEPS® FETECECTIN ngwa ngwa
Ibufe onye na - ebugharị ya, nke Aclecy College nke America kọwara, nke Acle Collegetricias na Gynecomiless na-enyefe tupu izu nke iri atọ na asaa nke ihe na-abụghị chromosimal perbination na-enweghị ọrịa. Ihe mgbaàmà nke nnyefe onye na - enye onye na - enye ya egwu, gbanwee nke ndọpụ uterine, ọbara ọgbụgba, ọghọm afọ, na ihe mgbochi. Ọnọdụ mgbanwe maka njirimara nke nnyefe nke onye na-atụnye ụtụ na-etinye akparamagwa nke Uterine ọrụ nyocha na arụmọrụ nke nyocha dijitalụ, nke na-ahapụ atụmatụ cervical akụkụ.
Nyochaa nnwale na-eme ngwa ngwa bụ nyocha immunochigraphic ule a ga-eji mee ihe maka ịchọpụta ogo maka njikọta FETAL FETAL na njirimara cervicovagine:
Dị mfe ojiji:Usoro nzọụkwụ na nnwale nnwale
Ngwangwa:naanị nkeji iri chọrọ otu onye ọrịa na-eleta
Ngwá ọrụ na-akwụghị ụgwọ:ụlọ ọgwụ na-egbochi ma ọ bụ ọnọdụ ụlọ ọgwụ nwere ike ịme ule a
Napụtara:ụlọ okpomọkụ (2 ℃ -30 ℃)
