OJI EJI EKWU
Nzọụkwụ siri ike^®Nnwale PROM bụ ule immunochromatographic atụgharịrị anya nke ezubere iji mee nchọpụta qualitative nke fibronectin nwa ebu n'afọ na nzuzo nzuzo cervicovaginal.Ọnụnọ nke fibronectin nwa ebu n'afọ na nzuzo nke cervicovaginal n'etiti izu 22, ụbọchị 0 na izu 34, ụbọchị 6 nke ime bụ.jikọtara ya na nnukwu ihe ize ndụ nke nnyefe tupu oge eruo.

MMEBI
Ọbịbịa tupu oge eruo, nke American College of Obstetricians and Gynecologists kọwara dị ka nnyefe tupu izu 37 nke afọ ime, bụ maka ọtụtụ n'ime ọrịa na-abụghị chromosomal perinatal ọrịa na ọnwụ.Mgbaàmà nke ịmụ nwa na-eyi egwu gụnyere mkpụkọ uterine, mgbanwe nke mpụ nke ikpu, ọbara ọgbụgba, azụ mgbu, ahụ erughị ala afọ, nrụgide pelvic, na cramps.Usoro nchoputa maka njirimara nke nnyefe tupu oge eruo gụnyere nlekota ọrụ uterine na arụmọrụ nke nyocha cervical dijitalụ, nke na-enye ohere nleba anya nke akụkụ cervical.E gosiputara ụzọ ndị a ka ọ dị oke, ebe ọ bụ na mgbasa cervical pere mpe (< 3 centimeters) na ọrụ uterine na-emekarị ma ọ bụchaghị nchọpụta nke nnyefe dị nso.Ọ bụ ezie na enyochala ọtụtụ ihe nrịbama biochemical, ọ nweghị nke anabatara nke ọma maka ojiji ụlọ ọgwụ bara uru.

Fibronectin nke nwa ebu n'afọ (fFN), isoform nke fibronectin, bụ glycoprotein nrapado dị mgbagwoju anya nwere ịdị arọ molekụla nke ihe dịka 500,000 daltons.Matsuura na ndị ọrụ na-arụkọ ọrụ akọwawo ihe mgbochi monoclonal a na-akpọ FDC-6, nke na-amata kpọmkwem III-CS, mpaghara na-akọwapụta ọdịdị nwa ebu n'afọ nke fibronectin.Nnyocha nke immunohistochemical nke placentae egosila na fFN bụetinyere na matrix extracellular nke mpaghara na-akọwa njikọ ahụakụkụ nke nne na nwa ebu n'afọ n'ime akpanwa.

Enwere ike ịhụ fibronectin nwa ebu n'afọ na nzuzo nke cervicovaginal nke ụmụ nwanyị n'oge ime ime site na iji ọgwụ mgbochi ọrịa monoclonal nke dabeere na immunoassay.Fibronectin fetal na-ebuli elu na nzuzo nke cervicovaginal n'oge mmalite ime ime mana ọ na-ebelata site na izu 22 ruo 35 n'ime afọ ime nkịtị.Ihe dị mkpa nke ọnụnọ ya na ikpu n'ime izu mbụ nke ime ime adịghị aghọta.Agbanyeghị, ọ nwere ike gosipụta uto nkịtị nke ọnụ ọgụgụ trophoblast pụrụ iche na placenta.Nchọpụta fFN na nzuzo cervicovaginal n'etiti izu 22, ụbọchị 0 na izu 34, a na-akọ na ime ime ụbọchị 6 na-ejikọta ya na nnyefe preterm na symptomatic na n'etiti izu 22, ụbọchị 0 na izu 30, ụbọchị 6 n'ime ụmụ nwanyị dị ime asymptomatic.

Ụkpụrụ
Nzọụkwụ siri ike^®Nnwale fFN na-eji ụcha immunochromatographic, teknụzụ na-erugharị capillary.Usoro nnwale ahụ chọrọ sọlfọ nke fFN site na swab ikpu site na ịgwakọta swab na Sample Buffer.Mgbe ahụ, a na-agbakwunye ihe nchekwa ihe agwakọtara na kaseti nlele nke ọma na ngwakọta na-akwaga n'akụkụ elu akpụkpọ ahụ.Ọ bụrụ na fFN dị na nlele ahụ, ọ ga-etolite mgbagwoju anya yana mgbochi mgbochi fFN bụ isi jikọtara na ụmụ irighiri ihe nwere agba.A ga-ejikọta mgbagwoju ahụ site na mgbochi fFN nke abụọ kpuchie na akpụkpọ ahụ nitrocellulose.Ọdịdị nke akara ule a na-ahụ anya yana akara akara ga-egosi nsonaazụ dị mma.

Ngwakọta ngwa

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ

MKPACHAPỤ ANYA
∎ Maka ọkachamara na-achọpụta ọrịa in vitro naanị.
∎ Ejila ụbọchị njedebe nke egosiri na ngwugwu ahụ.Ejila ule ma ọ bụrụ na akpa foil ya mebiri emebi.Ejighachila ule ọzọ.
■ Ngwa a nwere ngwaahịa sitere na anụmanụ.Ọmụma akwadoro maka mmalite na/ma ọ bụ ọnọdụ ịdị ọcha nke anụmanụ anaghị ekwe nkwa kpamkpam enweghị ihe nje na-efe efe.Ya mere, a na-atụ aro ka a na-emeso ngwaahịa ndị a dị ka ndị nwere ike ibute ọrịa, ma jikwaa ya na-edebe nchekwa nchekwa ndị a na-emebu (a etinyela ma ọ bụ kuku ume).
∎ Zenarị mmetọ nke ihe nlere anya site n'iji igbe nnakọta ihe ọhụrụ maka ụdịdị ọ bụla enwetara.
■ Jiri nlezianya gụọ usoro niile tupu ịme ule ọ bụla.
∎ erila, ṅụọ ihe ọṅụṅụ ma ọ bụ sịga n'ebe a na-edozi ihe nlele na ngwa.Jikwaa ụdịdị niile dị ka a ga-asị na ha nwere ihe na-efe efe.Leba anya n'akpachapụ anya siri ike megide ihe egwu microbiological n'ime usoro a niile wee soro usoro ọkọlọtọ maka mkpofu nke ọma.Yiri uwe nchebe dị ka uwe nyocha ụlọ nyocha, gloves enwere ike iwepu na nchedo anya mgbe a na-enyocha ihe nlele.
∎ Agbanwela ma ọ bụ gwakọta reagents site na nza dị iche iche.Ejikọtala mkpuchi karama ngwọta.
■ Iru mmiri na okpomọkụ nwere ike imetụta nsonaazụ.
∎ Mgbe usoro nyocha ahụ mechara, tụfuo swabs nke ọma mgbe ịmechara ya na 121ºC ma ọ dịkarịa ala nkeji iri abụọ.N'aka nke ọzọ, enwere ike ịgwọ ha na 0.5% sodium hypochloride (ma ọ bụ ịcha ọcha nke ụlọ) maka otu awa tupu atụfuo ya.Ekwesịrị ịtụfu ihe eji eme nnwale dịka iwu mpaghara, steeti na/ma ọ bụ gọọmenti etiti siri dị.
■ Ejila brushes cytology na ndị ọrịa dị ime.

Nchekwa na Nkwụsi ike
∎ Ekwesịrị idobe ngwa ahụ na 2-30C ruo mgbe ebibiela n'akpa mkpuchi mkpuchi.
■ Nnwale ahụ ga-adịrịrị n'ime obere akpa ahụ emechiri emechi ruo mgbe ejiri ya.
■ Ajụla oyi.
■ Ekwesịrị ịkpachapụ anya iji chebe ihe ndị dị na ngwa a pụọ ​​na mmetọ.Ejila ma ọ bụrụ na enwere ihe akaebe nke mmetọ microbial ma ọ bụ mmiri ozuzo.Mmetọ ndu nke akụrụngwa na-ekesa, arịa ma ọ bụ reagents nwere ike ibute nsonaazụ ụgha.

Mkpokọta PECIMEN na Nchekwa
∎ Jiri naanị Dacron ma ọ bụ Rayon tipped swabs nwere mkpanaka rọba.A na-atụ aro ka iji swab nke onye na-emepụta kits na-enye (Swabs adịghị na ngwa a, maka ozi ịtụ, biko kpọtụrụ onye nrụpụta ma ọ bụ onye nkesa mpaghara, nọmba katalọgụ bụ 207000).Emebebeghị ka swabs sitere na ndị na-ebubata ngwaahịa ndị ọzọ.A naghị atụ aro swabs na ndụmọdụ owu ma ọ bụ osisi osisi.
■ A na-enweta ihe nzuzo nke cervicovaginal site na fornix azụ nke ikpu.Ezubere usoro nchịkọta ka ọ dị nro.Nchịkọta siri ike ma ọ bụ ike siri ike, nke a na-ahụkarị maka omenala microbiological, achọghị.N'oge a na-enyocha ihe nlere anya, tupu nyocha ọ bụla ma ọ bụ ịmegharị cervix ma ọ bụ traktị ikpu, jiri nwayọọ tụgharịa n'ọnụ onye na-achọ ihe n'ofe azụ azụ nke ikpu maka ihe dịka 10 sekọnd iji nweta ihe nzuzo cervicovaginal.Mgbalị ndị na-esote imeju n'ọnụ onye ntinye nwere ike imebi ule ahụ.Wepu ngwa ngwa wee mee nnwale ahụ dịka enyere ya n'okpuru.
∎ Tinye swab n'ime tube mmịpụta, ọ bụrụ na enwere ike ịme ule ahụ ozugbo.Ọ bụrụ na ọ gaghị ekwe omume nnwale ozugbo, a ga-edobe ihe nlele onye ọrịa n'ime ọkpọkọ ebu njem kpọrọ nkụ maka nchekwa ma ọ bụ mbugharị.Enwere ike ịchekwa swabs ahụ maka awa 24 na ụlọ okpomọkụ (15-30 ° C) ma ọ bụ otu izu na 4 Celsius ma ọ bụ karịa ọnwa 6 na -20 Celsius.Ekwesịrị ikwe ka ụdịdị niile rute n'ime ụlọ nke 15-30ºC tupu nnwale.

Usoro
Weta ule, ụdị, ihe nchekwa na/ma ọ bụ njikwa n'ime ụlọ okpomọkụ (15-30°C) tupu eji ya.
∎ Tinye ọkpọkọ amịpụta dị ọcha n'ebe a họpụtara n'ebe a na-arụ ọrụ.Tinye 1ml nke ihe nchekwa mmịpụta na tube mmịpụta.
∎ Tinye swab n'ime tube.Gwakọta ihe ngwọta nke ọma site n'ịtụgharị swab ahụ n'ike n'akụkụ akụkụ tube ọ dịkarịa ala ugboro iri (mgbe mmiri na-emikpu).A na-enweta nsonaazụ kacha mma mgbe agwakọtara ihe atụ ahụ siri ike na ngwọta.
■ Wepụ mmiri mmiri dị ka o kwere mee site na swab site na ịpịa akụkụ nke tube mmịpụta na-agbanwe agbanwe ka ewepụrụ swab ahụ.Opekempe, 1/2 nke ihe nkpuchi ihe nlele ga-anọrịrị na tube maka ịkwaga capillary zuru oke ga-eme.Tinye okpu na tube ewepụtara.
Tụfuo swab ahụ n'ime akpa mkpofu ihe ize ndụ dị ndụ kwesịrị ekwesị.
∎ Ihe nlele ndị ewepụtara nwere ike idowe n'ime ụlọ okpomọkụ nkeji iri isii na-emetụtaghị nsonaazụ ule ahụ.
■ Wepụ ule ahụ na obere akpa ya mechiri emechi, ma tinye ya n'elu ala dị ọcha ma dịkwa larịị.Kpọọ ngwaọrụ ahụ na njirimara onye ọrịa ma ọ bụ njikwa.Iji nweta nsonaazụ kacha mma, a ga-eme nyocha ahụ n'ime otu awa.
∎ Tinye ntụsara ahụ 3 (ihe dị ka 100 µl) nke ihe nlele ewepụtara na tube Extraction na nlele nke ọma na cassette ule.
Zenarị ọnyà ikuku n'ụdị nke ọma (S), ma etinyekwala ngwọta ọ bụla na windo nlele.
Ka ule na-amalite ịrụ ọrụ, ị ga-ahụ ka agba na-aga n'ofe akpụkpọ ahụ.
∎ Chere ka eriri (s) nwere agba pụta.Ekwesịrị ịgụ nsonaazụ ya na nkeji ise.Atụgharịla nsonaazụ ya mgbe nkeji ise gachara.
Tụfuo ọkpọkọ ule ejiri mee ihe yana kasetị nwale n'ime akpa mkpofu ihe ize ndụ kwesịrị ekwesị.
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