Ezubere iji
Nzọụkwụ siri ike^®Ngwa ngwa ule Adenovirus (Feces) bụ ihe a na-ahụ ngwa ngwaimmunoassay maka nchọpụta qualitative presumptive nke adenovirus n'ime mmadụụdị fecal.Ezubere ngwa a ka ọ bụrụ ihe enyemaka na nyocha nke adenovirus
ọrịa.

Okwu mmalite
Enteric adenoviruses, nke kachasị Ad40 na Ad41, bụ ihe na-ebute afọ ọsịsan'ọtụtụ ụmụaka na-arịa nnukwu ọrịa afọ ọsịsa, nke abụọnaanị maka rotaviruses.Ọrịa afọ ọsịsa siri ike bụ ihe na-akpata ọnwụn'ime ụmụaka n'ụwa nile, karịsịa na mba ndị na-emepe emepe.Adenovirus ọrịaE kewapụrụ nje ndị na-akpata ọrịa n'ụwa nile, ma nwee ike ịkpata afọ ọsịsana ụmụaka n'afọ.A na-ahụkarị ọrịa na ụmụaka na-erubeghịafọ abụọ, mana achọpụtala na ndị ọrịa nọ n'afọ niile.Nnyocha na-egosi na a na-ejikọta adenoviruses na 4-15% nke ihe niileọrịa gastroenteritis viral n'ụlọ ọgwụ.

Nchọpụta ngwa ngwa na nke ziri ezi nke gastroenteritis metụtara adenovirus na-enyere akana iguzobe etiology nke gastroenteritis na njikwa ndị ọrịa metụtara ya.Usoro nyocha ndị ọzọ dị ka eletrọnịkị microscope (EM) naNgwakọ nucleic acid dị oke ọnụ ma na-arụkwa ọrụ ike.Nyereọdịdị na-amachi onwe ya nke ọrịa adenovirus, dị oke ọnụ naule na-akpa ike nwere ike ọ gaghị adị mkpa.

Ụkpụrụ
Ngwaọrụ nyocha ngwa ngwa Adenovirus (Feces) na-achọpụta adenovirussite na nkọwa anya nke mmepe agba na imewarara.Akwụsịla ọgwụ mgbochi adenovirus na mpaghara nnwale nkeakpụkpọ ahụ.N'oge ule, ihe nlere anya na-emeghachi omume na mgbochi adenovirusjikọtara ya na ihe ndị nwere agba ma tinye ya na pad sample nke ule ahụ.Ngwakọta ahụ na-akwaga na akpụkpọ ahụ site na capillary edinam ma na-emekọrịta ihena reagents na akpụkpọ ahụ.Ọ bụrụ na enwere adenovirus zuru oke na ụdị, aagba agba ga-etolite na mpaghara ule nke akpụkpọ ahụ.Ọnụnọ nke aagba agba na-egosi nsonaazụ dị mma, ebe enweghị ya na-egosi ihe ọjọọpụta.Ọdịdị nke otu agba agba na mpaghara nchịkwa na-eje ozi dị ka anjikwa usoro, na-egosi na oke nha nke ihe atụ adịlaagbakwunyere na akpụkpọ anụ ahụ emeela.

Usoro
Weta ule, ụdị, ihe nchekwa na/ma ọ bụ njikwa n'ime ụlọ okpomọkụ(15-30 Celsius C) tupu eji ya.
1. Nchịkọta ihe atụ na ọgwụgwọ mbụ:
1) Jiri ihe dị ọcha, kpọrọ nkụ maka nchịkọta ihe atụ.Nsonaazụ kacha mma ga-abụenwetara ma ọ bụrụ na a na-eme nyocha ahụ n'ime awa 6 mgbe nchịkọta.
2) Maka ihe nlere siri ike: Kpupu ma wepụ ihe ntinye tube dilution.BụrụKpachara anya ka ị ghara ịwụfu ma ọ bụ gbasaa ngwọta site na tube.Chịkọta ihe atụsite na ịtinye mkpanaka ngwa n'ime ma ọ dịkarịa ala 3 saịtị dị iche iche nkefeces ịnakọta ihe dị ka 50 mg nke nsị (dabere na 1/4 nke agwa).Maka ụdị mmiri mmiri: Jide pipette ahụ kwụ ọtọ, fecal aspirateụdị, wee bufee 2 tụlee (ihe dị ka 80 µL) n'ime mmiri.tube nchịkọta ihe atụ nwere ihe nchekwa mmịpụta.
3) Dochie ngwa ngwa n'ime tube ma kpochie okpu ahụ nke ọma.Bụrụkpachara anya ka ị ghara imebi n'ọnụ nke dilution tube.
4) Ma jijiji tube mkpokọta ihe nlere ike ka ị gwakọta ihe atụ naihe nkpuchi mmịpụta.Ihe atụ ndị a kwadebere na tube nchịkọta ihe atụEnwere ike ịchekwa ya maka ọnwa 6 na -20 Celsius ma ọ bụrụ na anwaleghị ya n'ime 1 awa ka emecharankwadebe.

2. Nnwale
1) Wepụ ule ahụ na obere akpa ya ejiri akara, tinye ya na yaelu dị ọcha, dị larịị.Jiri onye ọrịa ma ọ bụ njikwa kpọọ ule ahụnjirimara.Maka nsonaazụ kacha mma, a ga-eme nyocha ahụ n'ime otuawa.
2) Iji mpempe akwụkwọ mpempe akwụkwọ, gbajie ọnụ ọnụ nke dilution tube.Jidetube ahụ kwụ ọtọ ma fesaa 3 ntụ ntụ nke ngwọta n'ime ihe nlele ahụ nke ọma(S) nke ngwaọrụ ule.Zenarị ọnyà ikuku n'ụdị nke ọma (S), ma etinyekwala ya
ngwọta ọ bụla na windo nsonaazụ.Ka ule na-amalite ịrụ ọrụ, agba ga-akwaga n'ofe akpụkpọ ahụ.

3. Chere ka eriri (s) nwere agba pụta.Ekwesịrị ịgụ nsonaazụ ya na nkeji irinkeji.Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.

Mara:Ọ bụrụ na ihe atụ ahụ adịghị akwaga n'ihi ọnụnọ nke ahụ, centrifugeihe nlere anya ewepụtara dị n'ime vial ihe nchekwa mmịpụta.Anakọta 100µL nkedị elu, tinye n'ime ụdị nke ọma (S) nke ngwaọrụ nnwale ọhụrụ wee malite ọzọ, na-agbaso ntuziaka akọwara n'elu.

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